Validating protein biomarkers november

16 Dec

The bands were directly cut out of the gels, destained with 50% acetonitrile in 25 m M ammonium bicarbonate and dried in a speed vacuum concentrator.

They were separated by SDS-PAGE on a 10% gel followed by staining with Coomassie blue.

Two sets of urines from different cohorts were collected for discovery and validation set.

The discovery set composed of 16 urine samples from 16 patients with biopsy-proven 4 MCD, 4 MN, 4 FSGS, and 4 healthy controls.

In the discovery set, each individual sample was used for the patients with NS but pooled sample was analyzed for healthy controls.

Sixty one urine samples from 51 patients with biopsy-proven 13 MCD, 26 MN, 5 FSGS, 9 Ig AN and 8 healthy controls were included in validation set (Table ).